|
KMID : 1094720110160040706
|
|
Biotechnology and Bioprocess Engineering
2011 Volume.16 No. 4 p.706 ~ p.706
|
|
|
Improvement of fatty acid biosynthesis by engineered recombinant Escherichia coli
|
|
Lee Sun-Hee
Jeon Eun-Young
Yun Hyun-Shik
Lee Jin-Won
|
|
|
Abstract
|
|
|
|
The purpose of this research was to develop new strains of Escherichia coli with improved fatty acid biosynthesis. β-Ketoacyl acyl carrier protein synthase III (fabH) catalyzes the first step in the synthesis of fatty acids in parallel with acetyl-CoA carboxylase (accABC) and malonyl-CoA: acyl carrier protein transacylase (fabD) in Escherichia coli K-12 MG1655. The enzyme encoded by the fabH gene leads to an increase in the synthesis of short-chain-length fatty acids and a strong preference for acetyl-CoA, as it produces only straight chain fatty acids (SCFAs). It also seems to play a role in determining the type and composition of fatty acids produced. In this study, metabolically engineered strains of E. coli K-12 MG1655 containing fabH or accA::accBC::fabD or accA::accBC:: fabD::fabH gene-inserted expression vector (pTrc99A) were constructed. To observe the effects of overexpression, the production of malonic acid, a pathway intermediate, and fatty acids was analyzed. The resulting recombinant strains produced total lipids up to approximately 1.2 ∼ 1.6 fold higher than that of wild-type E. coli. The production of hexadecanoic acid was especially enhanced up to approximately 4.8 fold in E. coli SGJS13 as compared to E. coli SGJS11.
|
|
|
KEYWORD
|
|
|
Escherichia coli, fatty acids, fatty acid biosynthesis, acetyl-CoA carboxylase, β-Ketoacyl acyl carrier protein synthase III
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
 
|